Immunoassay using ascitic fluid

In a method for assaying an Ab in a liquid comprising the steps of:

• (a) adding to the liquid a known amount of inert carrier particles coated with Ag to the Ab, the particles being agglutinable on contact with both the Ab and euglobulin, and euglobulin to form a mixture;
• (b) incubating the mixture formed in step (a);
• (c) counting the number of unagglutinated particles;
• and
• (d) calculating thereby the amount of Ab in the sample, the improvement comprises using in step (a) euglobulin capable of binding Ab:Ag complexes at a pH above 8 and derived from the active fraction of mouse ascitic fluid.

In a method for analyzing a liquid for Ab:Ag complexes therein comprising the steps of:

• (a) adding to the liquid a known amount of inert carrier particles coated with a substance selected from the group consisting of IgG and IgM, the particles being agglutinable on contact with both Ab:Ag complex and euglobulin, and euglobulin to form a mixture;
• (b) incubating the mixture formed in step (a);
• (c) counting the number of unagglutinated particles and calculating thereby the amount of complex in the liquid;
• the improvement comprising using in step (a) euglobulin capable of binding Ab:Ag complexes at a pH above 8 and derived from the active fraction of mouse ascitic fluid.

In a method for assaying an Ag in a liquid comprising the steps of:

• (a) adding to the liquid a known amount of inert carrier particles coated with Ab to the Ag assayed, the particles being agglutinable on contact with both the Ag and euglobulin, and euglobulin to form a mixture;
• (b) incubating the mixture formed in step (a);
• (c) counting the number of unagglutinated particles;
• and
• (d) calculating thereby the amount of Ag in the sample;
• the improvement comprising using in step (a) euglobulin capable of binding Ab:Ag complexes at a pH above 8 and derived from the active fraction of mouse ascitic fluid.