Precursor indicator compositions
AssigneeMiles Laboratories, Inc.
InventorWilliam I. White
Life of this patent
Estimating the term…
Abstract
A composition, test device, method of making a test device and process for determination of an analyte in a liquid sample are disclosed. More particularly, there is provided a composition for detecting an analyte in a liquid sample comprising an analyte-responsive component and an indicator composition having a plurality of components comprising at least one nonresponsive precursor component and at least one component effective in a liquid milieu to alter said precursor component so as to release at least one substance effective to permit said indicator composition, in combination with said analyte-responsive component, to produce a detectable response to said analyte. The composition can optionally be incorporated with a carrier to form a device.
Claims27
A composition for detecting an analyte in a liquid sample comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to cleave the ester or amide linkage so as to release a substance effective to permit said indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte.
A device for detecting a sample analyte which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte.
A device for detecting a sample analyte which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage;
- (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- (3) a component resulting from the alteration of the precursor component;
- and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone.
A process for preparing a device for detecting a sample analyte which comprises the steps of:
- (a) incorporating a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage;
- (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- and thereafter
- (b) drying the carrier which has been so incorporated.
A process for preparing a device for detecting a sample analyte which comprises the steps of:
- (a) incorporating a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage;
- (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- (3) a component resulting from the alteration of the precursor component;
- and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone;
- and thereafter
- (b) drying the carrier which has been so incorporated.
A method for detecting an analyte in a sample which comprises the steps of:
- (a) contacting the sample with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a component effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- and thereafter
- (b) observing any detectable response.
A method for detecting an analyte in a sample which comprises the steps of:
- (a) contacting the sample with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- (3) a component resulting from the alteration of the precursor component;
- and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone;
- and thereafter
- (b) observing any detectable response.
A method for detecting an analyte in a sample which comprises the steps of:
- (a) contacting the sample with a device which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- and thereafter
- (b) observing any detectable response.
A method for detecting an analyte in a sample which comprises the steps of:
- (a) contacting the sample with a device which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage;
- (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte;
- (3) a component resulting from the alteration of the precursor component;
- and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone;
- and thereafter
- (b) observing any detectable response.
Dependencies are inferred from the claim text.