Composition of matter and process
AssigneeThe Upjohn Company
InventorsLester A. Dolak, LeRoy E. Johnson
Life of this patent
Estimating the term…
Abstract
Novel antibiotic U-60, 394 producible in a fermentation under controlled conditions using a biologically pure culture of the microorganism Streptomyces woolenses, Dietz and Li sp.n., NRRL 12113. This antibiotic is strongly active against various Gram-positive bacteria, for example, Staphylococcus aureus and Streptococcus hemolyticus. It is also strongly active against the Gram-negative bacterium Streptococcus pneumoniae. Thus, antibiotic U-60, 394 can be used in various environments to eradicate or control such bacteria.
Claims8
Antibiotic U-60,394, which is active against Gram-positive bacteria, and which in its essentially pure crystalline form has the following characteristics:
- (a) molecular weight of 391.08014 (high resolution mass spectrometry);
- (b) color and form of crystals:
- yellowish-green needles;
- (c) is insoluble in water, soluble in methanol and acetone with difficulty, and easily soluble in ethyl acetate, methylene chloride and dimethylsulfoxide;
- (d) a characteristic .sup.13 C-NMR spectrum as shown in FIG. 1 of the drawings;
- (e) a characteristic .sup.1 H-NMR spectrum as shown in FIG. 2 of the drawings;
- (f) a characteristic UV spectrum as shown in FIG. 3 of the drawings;
- (g) a characteristic infrared absorption spectrum when dissolved in a mineral oil mull as shown in FIG. 4 of the drawings;
- (h) a melting point of 265.degree. C. to 266.degree. C. with decomposition;
- and
- base addition salts thereof.
A process for preparing antibiotic U-60,394 which comprises cultivating Streptomyces woolensis Dietz and Li sp.n., having the identifying characteristics of NRRL 12113, in an aqueous nutrient medium under aerobic conditions until substantial antibiotic U-60,394 activity is imparted to said medium.
A process for recovering antibiotic U-60,394 from a fermentation beer which comprises:
- (a) filtering said beer to obtain filtered beer containing antibiotic U-60,394;
- (b) adjusting the pH of the filtrate to about 4.0;
- (c) extracting said filtrate with a solvent for U-60,394 to obtain an extract containing antibiotic U-60,394;
- (d) evaporating said extract to an aqueous concentrate;
- and
- (e) purifying said extract by chromatographic means to obtain essentially pure antibiotic U-60,394.
A process for recovering antibiotic U-60,394 from a fermentation beer which comprises:
- (a) filtering said fermentation beer to obtain filtered beer;
- (b) extracting said filtered beer with methylene chloride to obtain an extract containing antibiotic U-60,394;
- (c) evaporating said extract under reduced pressure to an aqueous concentrate;
- and
- (d) chromatographing said extract on silica gel using the solvent systems ethyl acetate:acetone (8:5) and then ethyl acetate:acetone:water (8:5:0.5) to obtain essentially pure antibiotic U-60,394.
A biologically pure culture of the microorganism Streptomyces woolensis Dietz and Li sp.n., having the identifying characteristics of NRRL 12113, said culture being capable of producing the antibiotic U-60,394 in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances.
Dependencies are inferred from the claim text.