Composition for the analysis of the alkaline phosphatase and method therefor
AssigneeIstituto Sieroterapico e Vaccinogeno Toscano "Sclavo" S.p.A.
InventorFranco Meiattini
Life of this patent
Estimating the term…
Abstract
A novel composition is disclosed for determining the activity of the alkaline phosphatase, which comprises a salt of p-nitrophenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol. The composition affords advantages over the known composition especially as regards sensitivity.
Claims5
A composition for the analysis of alkaline phosphatase, said composition consisting essentially of a salt of p-nitro-phenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol.
A method for the determination of non-placental alkaline phosphatase which comprises contacting a sample containing both placental and non-placental alkaline phosphatase to be tested with a composition consisting essentially of a salt of p-nitrophenylphosphoric acid, a salt of magnesium, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol and thereafter photometrically determining the amount of non-placental alkaline phosphatase.
Dependencies are inferred from the claim text.