Method of differential estimation of glutamic acid-oxalacetic acid transaminase isozyme
AssigneeEiken Chemical Co., Ltd.
InventorsHiroko Wada, Hiroyasu Teranishi
Life of this patent
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Abstract
A method is disclosed for differential estimation of glutamic acid-oxalacetic acid transaminase isozyme which comprises estimating m-GOT by: preparing an immuno conjugate by adding an anti-s-GOT obtained by immunizing a mammal with purified s-GOT as antigen extracted from the blood or the tissue of the mammal into the human serum; adding sensitized particles which are allowed to couple said anti-s-GOT antibody to insoluble particles; and completely absorbing said s-GOT in said human serum.
Claims4
A method for differential estimation of glutamic acid-oxalacetic acid transaminase (GOT) isozyme in human serum which comprises determining the m-GOT fraction by preparing an immuno conjugate thereof by adding anti-s-GOT antibody obtained by immunizing a non-human animal with a purified s-GOT antigen extracted from the blood or tissue of said mammal, adding anti-s-GOT serum and said immuno conjugate into said human serum;
- adding sensitized particles capable of coupling said anti-s-GOT antibody to insoluble particles, thereby absorbing completely said s-GOT in the human serum;
- separating said absorbed s-GOT from said m-GOT fraction, and determining the level of said m-GOT.
Dependencies are inferred from the claim text.