Polypeptide derivatives
AssigneeKenneth Wingrove
InventorsRobert B. Johnston, James L. Balk, John T. Pelton
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Abstract
Compounds comprising polypeptide derivatives of the formula ##STR1## wherein R.sub.1 is the amino acid sequence of the polypeptide minus the terminal amino acid; R is the side chain group determining the identity of the terminal amino acid; and Z is ##STR2## or --CH.sub.2 X wherein X is Cl, Br, or I; are provided. Precursor peptides are peptide hypothalamic releasing and inhibitory factors, opiate peptides, and fragments thereof. The derivatives act as antagonists or agonists to the precursor peptides and are particularly useful in bioassay procedures.
Claims14
A biologically active derivative of a precursor carboxyl-terminal polypeptide comprising a sequence of .alpha.-amino acid residues terminated at one end thereof with a terminal carboxyl group and at the other end thereof with a terminal amino group, said precursor polypeptide being selected from the group consisting of the free acid forms of (a) polypeptide hypothalamic releasing factors, (b) polypeptide hypothalamic inhibitory factors, (c) polypeptide enkephalins, and (d) polypeptide fragments thereof each consisting of a biologically-active polypeptide segment of one of said precursor polypeptides, said derivative comprising said precursor polypeptide wherein the hydroxyl group of the terminal carboxyl group is replaced by --CH--N.sub.2 or --CH.sub.2 X, wherein X is Cl, Br, or I.
Dependencies are inferred from the claim text.