Method of selectively increasing yield and purity of certain cryoprecipitate proteins by heating
OwnerEdward Shanbrominventor
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Abstract
AHF and CIg yield and purity in cryoprecipitate concentrate are improved by suspending said cryoprecipitate concentrate in aqueous solution and then subjecting to a heat treatment step at a temperature of from about 45.degree. C. to about 56.degree. C. for a period of from about one minute to about sixty minutes to thereby selectively thermally precipitate the contaminating proteins without substantial loss or AHF of CIg activities and recovering the purified AHF on CIg from the precipitate of contaminating proteins.
Claims9
The method of improving the yield and purity of a substance selected from the group consisting of AHF and CIg in a cryoprecipitate concentrate of said substance comprising suspending said cryoprecipitate concentrate in aqueous solution and subjecting to heat treatment at a temperature of from about 45.degree. C. to about 56.degree. C. for a period of from about one to about sixty minutes to thereby selectively thermally precipitate contaminating proteins without substantial loss of said AHF or CIg and recovering the purified AHF or CIg by separation from the precipitate of contaminating proteins.
Dependencies are inferred from the claim text.