Recording transmission and emission spectra
AssigneeFarrand Optical Co., Inc.
InventorEli A. Kallet
Life of this patent
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Abstract
A first monochromator includes a diffraction grating that is adapted for occlusion by a mirror. When the mirror occludes the grating, a white light input is transmitted through the first monochromator and through a sample under test, thereby causing a transmission of non-absorbed light from the sample. When the grating is unoccluded, monochromatic light is transmitted from the first monochromator so that a selected bandwidth of fluorescence excitation energy is incident on the sample. Fluorescence emission from the sample is diffracted by a second monochromator which provides diffracted light which is recorded by a vidicon.
Claims4
Apparatus for providing transmission and fluorescent emission data from a sample comprising:
- a light source having a first mode of operation in which monochromatic light is directed toward a first side of said sample, and a second mode of operation in which white light is directed toward the first side of said sample;
- optical means having a first configuration for directing light transmitted from the light source through the sample back toward a second side of the sample, and a second configuration for directing light transmitted from the light source through the sample back to the side of the sample opposite the first side;
- and
- detector means for detecting the intensity of light emitted from the side of the sample opposite the second side.
Dependencies are inferred from the claim text.