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US 4,304,866 AGranted
Transplantable sheets of living keratinous tissue
AssigneeMassachusetts Institute of Technology
InventorsHoward Green, Olaniyi Kehinde
Life of this patent
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Abstract
A method of producing transplantable sheets of living keratinous tissue by culturing keratinocytes in a culture vessel and subsequently enzymatically detaching a sheet of keratinous tissue employing an enzyme, such as Dispase, is disclosed herein.
Claims5
A method of producing transplantable sheets of living keratinous tissue, comprising:
- a. culturing keratinocytes in a culture vessel under conditions whereby a sheet of keratinous tissue is formed upon the surface of the vessel, and
- b. incubating said sheet of keratinous tissue in the presence of a neutral protease under conditions sufficient to enzymatically detach the sheet of keratinous tissue from the surface of the vessel without disaggregating said sheet.
A method for treating defects in the epithelium of a mammal, comprising:
- a. obtaining living epidermal cells from said mammal;
- b. culturing said epidermal cells in a culture vessel to form a sheet of epidermal tissue in said vessel;
- c. incubating said sheet of epidermal tissue in the presence of a neutral protease under conditions sufficient to enzymatically detach said tissue from the culture vessel without disaggregating the epidermal cells;
- and,
- d. transplanting detached epidermal tissue onto the defect of said mammal.
2 more elements
Dependencies are inferred from the claim text.