Process for the production of hybrid bacteria
AssigneeGesellschaft fur Biotechnologische Forschung mbH
InventorsJohn Collins, Barbara Hohn
Life of this patent
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Abstract
The present invention is directed to a process for the production of hybrid bacteria, characterized in that (a) a hybrid plasmid having only one cos site of a lambda or a lambdoid phage is produced from (1) a bacterial plasmid of not more than 21 Megadaltons and having only one cos site of a lambda or a lambdoid phage and (2) a foreign DNA fragment, (b) the resulting hybrid plasmid is packaged with the lysate of a lambda or lambdoid phage and (c) the packaging product is transduced into Escherichia coli whereby hybrid bacteria are formed.
Claims4
Process for the production of hybrid bacteria, characterized in that
- (a) a hybrid plasmid having only one cos site of a lambda or a lambdoid phage is produced from (1) a bacterial plasmid of not more than 21 Megadaltons and having only one cos site of a lambda or a lambdoid phage and (2) a foreign DNA fragment,
- (b) the resulting hybrid plasmid is packaged with the lysate of a lambda or lambdoid phage and
- (c) the packaging product is transduced into Escherichia coli whereby hybrid bacteria are formed.
Dependencies are inferred from the claim text.