Method for increasing the diacetyl production of a diacetyl-producing bacteria
AssigneeThe Procter & Gamble Company
InventorJohn A. Troller
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Abstract
A method for increasing the diacetyl production of a diacetyl-producing bacteria such as Streptococcus diacetylactis. Glycerol or sucrose as humectants are added to an aqueous nutrient medium such as milk having a 2% butterfat content which contains a diacetyl precursor such as sodium citrate. The bacteria are then inoculated into the humectant-containing nutrient medium. The humectant lowers the a.sub.w value of the nutrient medium so as to increase the diacetyl production of the bacteria when incubated at a temperature of from 28.degree. to 37.degree. C.
Claims18
A diacetyl-producing composition, comprising:
- (a) an aqueous nutrient medium having a pH of about 4.5 to 7.0 and containing a metabolizable amount of diacetyl precursor;
- (b) a diacetyl-producing bacteria selected from the group consisting of S. diacetylactis, S. cremoris, S. lactis and mixtures thereof;
- and
- (c) sucrose in an amount sufficient to lower the a.sub.w value of said nutrient medium to from about 0.95 to 0.99 and to increase the diacetyl production of said bacteria per volume of said nutrient medium.
A method for increasing the diacetyl production of a diacetyl-producing bacteria selected from the group consisting of S. diacetylactis, S. cremoris, S. lactis and mixtures thereof, the bacteria being inoculated into an aqueous nutrient medium having a pH of from about 4.5 to 7.0 and containing a metabolizable amount of a diacetyl precursor, said method comprising the steps of:
- (a) incorporating in the nutrient medium prior to inoculation of the bacteria a humectant selected from the group consisting of glycerol, sucrose and mixtures thereof in an amount sufficient to lower the a.sub.w value of the nutrient medium to from about 0.95 to 0.99 and to increase the diacetyl production of the bacteria per volume of the nutrient medium;
- and
- (b) incubating the inoculated nutrient medium after step (a) at a temperature of from about 28.degree. to 37.degree. C. to produce the diacetyl.
Dependencies are inferred from the claim text.