Process of producing antibiotic SM-173B with Streptomyces chromofuscus

Abstract

A novel antibiotic having the ascribed formula ##STR1## is provided. They physical characteristics are as follows: (a) Elementary analysis: Calculated (for C.sub.20 H.sub.16 O.sub.7): C,65.22; H,4.35%. Found: C,64.60; H,4.34%. (b) Molecular weight (mass-spectrometric): 368. (c) Melting point: 225.degree.-226.degree. C. (d) Specific rotation: [.alpha]sub.D .sup.20 =+123.5.degree. (c=0.2 in chloroform). (e) Ultraviolet absorption spectrum: .lambda..sub.max .sup.methanol =235 nm (.epsilon.=28600); 262 nm (.epsilon.=21900); 434-435 nm (.epsilon.=11300); .lambda..sub.max .sup.0.1-N NaOH/methanol =246 nm (.epsilon.=22400); 256 nm (.epsilon.=23200); 523-524 nm (.epsilon.=9600). (f) Infrared absorption spectrum: 3500, 1710, 1672, 1628, 1600, 1575, 1472, 1452, 1420, 1390, 1295, 1255, 1218, 1200, 1182, 1105, 1025, 1005, 962, 938, 848, 765, 752, 730 cm.sup.-1. (g) Solubility: Soluble in methanol, ethanol, butanol, acetone, chloroform, ethyl acetate and in water under alkaline conditions; poorly soluble in n-hexane; insoluble in petroleum ether and water. (h) Color reactions: Positive with sulfuric acid and iodine; negative with ninhydrin and anisaldehyde. (i) NMR spectrum in DC.Cl.sub.3 : 1.42 ppm (3H, singlet), 3.02 ppm (1H, quartet), 3.43 ppm (3H, singlet), 3.62 ppm (1H, triplet), 3.64 ppm (1H, singlet), 3.93 ppm (1H, triplet), 7.31-3.41 ppm (4H, olefinic protons), 12.0 ppm (1H, singlet), 12.5 ppm (1H, singlet). Also provided is a process for the antibiotic's production.

Dependencies are inferred from the claim text.