Process for production of erythropoietin
AssigneesAjinomoto Co., Inc., Japan Chemical Research Co., Ltd.
InventorsKenji Takezawa, Hajime Hiratani
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Abstract
The invention provides a novel process for the production of erythropoietin, which is a promising medicine for curing anemia, from human urine containing the same. The inventive method comprises adjusting the pH value of the urine in the range from 6 to 8, if the pH value of the urine is out of this range, and contacting the thus pH-controlled urine with a specific adsorbent so as that the erythropoietin is selectively adsorbed on the adsorbent. The adsorbed erythropoietin is then eluted out by use of an eluant solution to give the product in a partially purified form.
Claims3
A process for the production of purified erythropoietin from human urine which comprises the steps of
- (a) adjusting the value of pH of the urine in the range from 6 to 8, if the pH of the urine is out of this range,
- (b) contacting the urine having the thus controlled pH value with an adsorbent selected from the group consisting of a polystyrene-based porous adsorbent resin, chitosan and diatomaceous earth whereby the erythropoietin is adsorbed on the adsorbent,
- (c) separating the adsorbent having adsorbed the erythropoietin from the depleted urine, and
- (d) eluting the erythropoietin out of the adsorbent.
Dependencies are inferred from the claim text.