Modified living canine parvovirus vaccine
AssigneeCornell Research Foundation, Inc.
InventorsLeland E. Carmichael, Max J. G. Appel, Douglas D. McGregor
Life of this patent
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Abstract
A safe, efficacious vaccine for protecting dogs against infection caused by canine parvovirus is produced by prolonged serial passages in non-oncogenic cell lines. One embodiment includes the use of sub-optimal temperatures (below that normal for the dog) during passaging.
Claims18
A method of protecting dogs from infection caused by canine parvovirus comprising inoculating an animal with modified living canine parvovirus vaccine (CPV) prepared in accordance with a procedure which comprises taking a seed of a virulent CPV strain and serially passaging the strain in non-oncogenic cell cultures at a sub-optimal temperature at which CPV growth occurs until a non-virulent, immunizing virus is produced.
A modified live vaccine for protecting dogs against infection caused by canine parvovirus produced by a process comprising the steps of taking a seed of a virulent CPV strain and serially passaging the strain in non-oncogenic cell cultures at a sub-optimal temperature at which CPV growth occurs with periodic tests until a non-virulent strain is produced.
The method of producing a modified live vaccine for protecting dogs against canine parvovirus comprising the steps of:
- a. Starting with a seed of virulent CPV;
- b. Serially passaging the virus in non-oncogenic cell cultures at a sub-optimal temperature at which CPV growth occurs;
- c. Periodically testing the passaged virus for virulence until a non-virulent virus is produced.
Dependencies are inferred from the claim text.