Cholera vaccine

A process for the preparation of a particulate material capable of inducing immunity and inducing the synthesis of antibodies to cholera toxin when administered to a living organism that possesses an immunity defense system which comprises support particles provided with a first coating of a ganglioside having an affinity for cholera toxin and a second coating of cholera toxin attached to said ganglioside coating, said support particles being selected from red blood corpuscles, inactivated bacteria particles, an organic polymer capable of giving a stable suspension in water, carbon particles or porous mineral particles coated with a polysaccharide polymer or with an oxidative scission product thereof, crosslinked or not comprising in a first stage affixing said ganglioside onto said support particles to provide said first coating on said support particles and in a second stage attaching said cholera toxin onto said ganglioside coating, the amount of attached cholera toxin being such that the attachment sites of said ganglioside are not saturated with said cholera toxin.

An parenterally administrable medicine for inducing an immunity response to cholera toxin comprising a dosage of a particulate material capable of inducing immunity and inducing the synthesis of antibodies to cholera toxin consisting essentially of support particles selected from inactivated bacteria particles and soluble polysaccharides provided with a first coating of a ganglioside having an affinity for cholera toxin and a second coating of cholera toxin attached to said ganglioside coating, said dosage being effective to induce said immunity response.

A medicine for inducing an immunity response to cholera toxin comprising a dosage of a particulate material capable of inducing immunity and inducing the synthesis of antibodies to cholera when administered to a living organism that possesses an immunity defense system which consists essentially of support particles provided with a first coating of a ganglioside having an affinity for cholera toxin and a second coating of cholera toxin attached to said ganglioside coating, said dosage being effective to induce said immunity response.

A process for inducing the formation of cholera antitoxin antibodies comprising injecting into an animal a particulate material comprising support particles provided with a first coating of a ganglioside having an affinity for cholera toxin and a second coating of cholera toxin attached to said ganglioside coating, said support particles being selected from red blood corpuscles, inactivated bacteria particles, an organic polymer capable of giving a stable suspension in water, carbon particles or porous mineral particles coated with a polysaccharide polymer or with an oxidative scission product thereof, crosslinked or not so as to induce the synthesis of said cholera antitoxin antibodies and subsequently isolating the said resulting antibodies.