Removal of interferents in analytical assays in a two phase interferent-removal zone
AssigneeEastman Kodak Company
InventorsSang H. Kim, Richard W. Spayd
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Abstract
Novel elements for the analysis of liquids which contain interferents for an analyte assay are described. Interference is reduced in multizone elements by the presence of an interferent-removing zone between the point of introduction of the liquid and the assay-indicator zone wherein the interferent-removing zone comprises a discontinuous phase within a continuous phase. An interferent-removing material is present in the discontinuous phase and the continuous phase is permeable to the analyte, its products and/or the liquid being analyzed.
Claims53
In an element for analysis of a liquid containing an analyte wherein an interferent is removed from the assay of the analyte, said element comprising at least two zones wherein at least one zone comprises an interferent-removal zone, and at least one additional zone comprises an assay indicator zone, the improvement wherein the zone for interferent-removal comprises a discontinuous liquid phase within a continuous phase wherein the discontinuous liquid phase comprises a material for extracting an interferent to the assay of said analyte and the continuous phase is permeable to said liquid under analysis, the analyte or an interaction product, said interferent-removal zone being located between the assay indicator zone and the point of introduction of the liquid.
A method for the analysis of a liquid containing an analyte in a multizone analytical element wherein at least one zone is an interferent-removal zone and at least one zone is an assay indicator zone, said method comprising:
- (a) contacting the element with said liquid;
- and
- (b) detecting in said indicator zone after a predetermined time the detectable change produced in said element,
- the improvement wherein said interferent-removal zone comprises a discontinuous liquid phase within a continuous phase, said discontinuous liquid phase comprising a material for extracting the interferent to the assay of said analyte and the continuous phase being permeable to said liquid under analysis, the analyte or an interaction product, said interferent-removal zone being located between the assay indicator zone and the point of contact of the element with said liquid.
Dependencies are inferred from the claim text.