US 4,302,905 AGranted

Growing cells of plants in a multi-media hydroponic environment

OwnerVesper A. Vaseeninventor

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Abstract

Living cells of plants, such as Thallophyta and Embryophyta are grown at an accelerated rate in a multi-medium hydroponics mixture consisting of a carbon dioxide gas impregnated inert, nonionic, non-aggressive, liquid medium which is non-miscible with water; and an aqueous hydroponic nutrient liquid medium; as compared to the single medium nutrient aqueous liquid medium. The process consists of growing clone cells, or callus, in a mechanically mixed suspension of a nutrient hydroponic liquid, made up of water and selected soluble mineral nutrients; and supplementing the growing cells need of carbon, the principal cell mass, with carbon dioxide carried dissolved as a non-ionic gas in an inert, non-hypertonic, non-hypotonic liquid; rather than use of "sugar". Mixing the nutrient aqueous hydroponic liquid and the inert carrier liquid provides not only the exchange of carbon dioxide to the cells, but receives from the cell and the aqueous liquid the cell evolved oxygen; thus expediting further the healthy growth of live plant cells. Live plant clone cells are separated from the liquids, as product, and the liquids replenished for recycle use.

Claims1

Dependent claims

I claim a method of growing clones and diversifying cells of plants by use of a multi-media hydroponics consisting of an aqueous nutrient solution in conjunction with an inert, non-ionic liquid saturated with non-ionic carbon dioxide gas;

consisting of:
growing laboratory cultures of acclimated callus or diversifying plant cells, by the method of,
dissolving predetermined quantities of mineral nutrients salts in a sterile aqueous solution, thereby,
providing a nutrient solution conducive to plant cell growth,
injecting laboratory cultured starter or seed cells into the nutrient solution,
saturating an inert, non-ionic liquid, preferably a polyorganosiloxane or flourocarbon with carbon dioxide gas,
mixing the carbon dioxide saturated inert liquid with the aqueous cell suspension to provide via the absorbed carbon dioxide, carbon sufficient for predetermined cell growth quantity,
adjusting and retaining the temperature of the multi-media mixture between 20.degree. C. and 30.degree. C.,
mixing the multi-media suspension at a rate not mechanically destructive of cells,
providing photosynthesis light energy at an intensity of from 100 to 2,000 foot candles at the liquid surface,
removing carbon dioxide spent inert liquid and replacing said liquid with renewed carbon dioxide saturated liquid periodically, as required to satisfy mitosis and separating and removing cells from the liquid.

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