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US 4,302,544 AGranted
Asporogenous mutant of B. subtilis for use as host component of HV1 system
AssigneeUniversity of Rochester
InventorsFrank E. Young, Gary A. Wilson, Susan L. Mottice
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Abstract
Asporogenous mutant B. subtilis RUB 331 (ATCC 31578) and a process for using such mutant are disclosed. The asporogenous mutant desirably reverts to sporeformers with a frequency not greater than 10.sup.-7 reversions per bacterium per generation and meets the requirements of the NIH "Guidelines for Research Involving Recombinant DNA Molecules" for a B. subtilis host component of a Host-Vector 1 system.
Claims1
A biologically pure culture of asporgenous B. subtilis RUB 331 (ATCC 31578) suitable for use as a host component in a host-vector system having the following characteristics:
- translucent phenotype on tryptose blood agar plates but not on Spizizen's minimal agar supplemented with glucose;
- a frequency of transformation with linear or covalently closed circular DNA of up to 2 percent;
- lyses in a complex medium;
- viability reduced to 0 CFU/ml after drying at room temperature for about 12 hours and a frequency of reversion to sporeformers of less than 10.sup.-7, under conditions of minimal aeration.
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Dependencies are inferred from the claim text.