Reagent and method for the determination of peroxidase
AssigneeBoehringer Mannheim GmbH
InventorsKarl-Dietrich Gundermann, Karl Wulff, Fritz Staehler, Hans-Ralf Linke
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Abstract
The present invention provides a method for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide of the formula ##STR1## is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.
Claims20
Method for the determination of peroxidase in a sample, which method comprises contacting the sample with a peroxy compound selected from hydrogen peroxide or an organic hydroperoxide and 7-dimethyl-aminonaphthaline-1,2-dicarboxylic acid hydrozine of the formula ##STR3## and measuring the emission of light as a measure of the peroxidase initially present in the sample.
Reagent for the determination of peroxidase comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.
Dependencies are inferred from the claim text.