Method for concentrating and purifying antihemophilic factor or factor VIII
AssigneeInstitut Merieux
InventorsJean F. Pla, Jacques C. Liautaud
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Abstract
A method is disclosed for concentrating and purifying antihemophilic factor (AHF or Factor VIII), a cryoprecipitate is collected from frozen human plasma and cold washed with pyrogen-free distilled water or water saline. The washed cryoprecipitate is extracted with pyrogen-free distilled water, the prothrombin complex is removed and the fibrinogen is precipitated. The remaining purified solution is ultrafiltered to form a highly concentrated Factor VIII solution.
Claims9
In a method of concentrating and purifying Factor VIII comprising the steps of (1) collecting cryoprecipitate from frozen human plasma, (2) extracting the cryoprecipitate, (3) removing prothrombin complex, (4) precipitating fibrinogen and thereafter (5) ultra-filtering the remaining solution to form a purified Factor VIII concentrate,
- the improvement consisting of cold washing the cryoprecipitate before extraction step (2) with an aqueous saline solution.
In a method of concentrating and purifying Factor VIII consisting essentially of the steps of (1) collecting cryoprecipitate from frozen human plasma, (2) extracting the cryoprecipitate in pyrogen-free water at room temperature at a pH of about 7, (3) adding aluminum hydroxide to adsorb prothrombine complex and adjusting the pH to about 7, (4) cooling the mixture to a temperature of from about +1.degree. to about +7.degree. C. and thereafter (5) removing the thus-formed fibrinogen-containing precipitate to obtain a Factor VIII concentrate,
- the improvement comprising steps of cold washing the cryoprecipitate before extraction steps (2) with an aqueous saline solution, and by ultra-filtering the Factor VIII concentrate obtained in step (3).
Dependencies are inferred from the claim text.