Purification of gammaglobulin derivative
AssigneeJuridical Foundation The Chemo-Sero-Therapeutic Research Institute
InventorsAkinobu Funatsu, Shuzoh Oyama, Yoshinori Akimoto, Komei Ohashi
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Abstract
Process for the production of a highly purified S-sulfonated gammaglobulin by treating an S-sulfonated gammaglobulin obtained by sulfonating a conventional gammaglobulin with an ion exchanger in a buffer solution for development, and thereby absorbing single molecular S-sulfonated gammaglobulin thereto, and eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution. This process can give the desired S-sulfonated gammaglobulin having a high content of single molecule and an extremely small anticomplementary activity (e.g. CH.sub.50 of 10% or less) and also an excellent shaking stability from the conventional gammaglobulin in an industrial scale.
Claims9
A process for the production of a purified S-sulfonated gammaglobulin which comprises treating an S-sulfonated gammaglobulin with an anion exchanger in a buffer solution for development having a pH level of 7 to 8 and an ionic strength of 0.01 to 0.15 and thereby absorbing single molecular S-sulfonated gammaglobulin on the anion exchanger, and then eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution.
A process for the production of a purified S-sulfonated gammaglobulin which comprises treating an S-sulfonated gammaglobulin with a cation exchanger in a buffer solution for development having a pH level of 4 to 6.5 and an ionic strength of 0.01 to 0.1 and thereby absorbing single molecular S-sulfonated gammaglobulin onto the cation exchanger, and then eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution.
A process according to any one of claim 5, 8 or 9, wherein a buffer solution having a pH level of 6 to 9 and an ionic strength of higher than that of the buffer solution for development is used as the buffer solution for elution.
A process for separating single molecules from aggregated molecules of S-sulfonated gammaglobulin which comprises preferentially absorbing the said single molecules on an ion exchanger from a buffered solution having a pH which is compatable with the ion exchanger and eluting the ion exchanger to remove the absorbed gamma globulin.
Dependencies are inferred from the claim text.