Platelet reference control
AssigneeWarner-Lambert Company
InventorsJames E. Turner, Jr., Michael B. Kenoff
Life of this patent
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Abstract
This invention describes a novel method for preparing a stable suspension of individual blood platelets for use as a control in both manual and automatic platelet counting procedures.
Claims8
A method of preparing a stable suspension of unaggregated blood platelets which comprises adding dichromate salt to an amount of platelet rich plasma and adding glutaraldehyde to the dichromatic-platelet rich plasma mixture to a final concentration of between 0.2-0.3% (w/v) of dichromate salt and between 0.05-0.15% (w/v) glutaraldehyde and incubating the resultant suspension at a temperature of 30.degree.-40.degree. C. for an effective period of time.
A method of preparing a stable suspension of unaggregated blood platelets which comprises:
- i. Adding a dichromate salt to a platelet rich plasma and adding glutaraldehyde to the dichromate-platelet rich plasma to a final concentration of between 0.2-0.3% (w/v) of dichromate salt and between 0.05-0.15% (w/v) glutaraldehyde;
- ii. Allowing the suspension so prepared to react for 30 to 180 minutes at a temperature of 30.degree. C. to 40.degree. C.;
- iii. Removing the fixed platelets;
- and
- iv. Resuspending the platelets in a solution selected from the following group consisting of:
- (a) physiological saline containing serum albumin at a concentration of 1.25% to 7.5% (w/v);
- (b) dimethylsulfoxide diluted with physiological saline to a concentration of 2.5% to 20% (v/v);
- and (c) physiological saline.
Dependencies are inferred from the claim text.