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US 4,301,250 AGranted
Method of producing hepatitis B surface antigen
AssigneeMerck & Co., Inc.
InventorsWilliam J. McAleer, Henry Z. Markus
Life of this patent
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Abstract
Hepatitis B surface antigen (HB.sub.s Ag) is produced in vitro in high titer and purity from tissue cultures of cells that shed HB.sub.s Ag by growing the cells with a first incubation at elevated temperature followed by a second incubation at a lower elevated temperature.
Claims9
A method for preparing hepatitis B surface antigen which comprises growing cells which shed hepatitis B surface antigen in the presence of a nutrient medium in a first growth stage at a temperature of about 37.degree. C. and a second growth stage at a temperature of from about 30.degree. to 34.degree. C.
Dependencies are inferred from the claim text.