Process for chenodeoxycholic acid production
AssigneeHoffmann-La Roche Inc.
InventorsCarl Despreaux, Thomas A. Narwid, Norberto J. Palleroni, Milan R. Uskokovic
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Abstract
A multi-step synthesis of chenodeoxycholic acid from 3-keto-bisnorcholenol, a compound readily obtained from the abundant plant sterol .beta.-sitosterol, is described. A key step in the synthesis is the stereoselective microbial introduction of the 7-alpha hydroxy group into 3-keto-bisnorcholenol.
Claims5
A process for the preparation of 7-alpha-hydroxy-3-keto-bisnorcholenol which process comprises 7-alpha-hydroxylating 3-keto-bisnorcholenol by contacting said 3-keto-bisnorcholenol with a microorganism selected from the group consisting of Botryodiplodia theobromae IFO 6469, DSM 62-678, DSM 62-679;
- Lasiodiplodia theobromae ATCC 28570;
- Botryosphaeria ribis ATCC 22802, B. berengeriana ATCC 12557, B. rhodina CBS 374.54, CBS 287.47 and CBS 306.58, said microorganism being in the form of a culture broth, mycelia or an enzyme extract thereof.
Dependencies are inferred from the claim text.