Heparin preparation
AssigneeRiker Laboratories, Inc.
InventorRobert D. Rosenberg
Life of this patent
Estimating the term…
Abstract
A heparin preparation which exhibits elevated anticoagulant activity and a process for producing the preparation. Conventional heparin exhibiting characteristic anticoagulant activity and molecular heterogeneity is incubated with antithrombin-heparin cofactor extracted from plasma. A portion of the heparin forms a complex with the cofactor. The uncomplexed heparin fraction is then separated from the complexed fraction, and the complexed fraction is broken down to produce cofactor and an active form of heparin.
Claims7
A process for producing heparin characterized by elevated anticoagulant activity, said process comprising the steps of:
- A. providing a heparin preparation of animal tissue origin which exhibits molecular heterogeneity and anticoagulant activity;
- B. providing AT cofactor extracted from mammalian plasma;
- C. incubating the heparin preparation with the AT cofactor to complex a portion of the heparin with the cofactor;
- D. separating a complex of heparin and cofactor from the remainder of the heparin preparation;
- and,
- E. separating the cofactor from the complex to produce heparin having improved anticoagulant activity.
A potentiated heparin preparation prepared from heparin of animal tissue origin which has been complexed with AT cofactor, separated from heparin uncomplexed with AT cofactor, and separated from the heparin AT cofactor complex to yield a heparin preparation having greater anticoagulant activity than heparin from the animal tissue origin.
A process for inhibiting coagulation of blood, said process comprising the steps of:
- A. providing a heparin preparation of animal tissue origin which exhibits molecular heterogeneity and anticoagulant activity;
- B. providing AT cofactor extracted from mammalian plasma;
- C. incubating the heparin preparation with the AT cofactor to complex a portion of the heparin with the cofactor;
- D. separating a complex of heparin and cofactor from the remainder of the heparin preparation;
- E. separating the cofactor from the complex to produce heparin having improved anticoagulant activity;
- and,
- F. mixing the potentiated heparin with blood.
Dependencies are inferred from the claim text.