Ubiquitary tissue protein PP.sub.8

An isolated, enriched tissue protein obtainable by fractionating an aqueous organ extract, the amino-acid composition of said tissue protein being:

• ______________________________________ Variation coefficient Amino acid Mole % (%) ______________________________________ Lysine 7.08 3.21 Histidine 1.49 22.07 Arginine 3.56 3.86 Aspartic acid 10.32 6.79 Threonine 6.08 0.96 Serine 7.88 3.99 Glutamic acid 12.97 3.48 Proline 2.87 4.84 Glycine 6.64 2.19 Alanine 6.41 0.65 Cystine/2 2.00 17.75 Valine 5.91 3.81 Methoionine 4.67 14.45 Isoleucine 3.10 2.01 Leucine 9.29 3.58 Tryosine 1.65 14.40 Phenylalanine 6.67 7.43 Tryptophan 1.41 2.13 ______________________________________
• and said tissue protein having
• (a) a protein proportion of 96.+-.3%,
• (b) a carbohydrate content of 4.1.+-.0.95%, of which 3.15.+-.0.5% are hexoses, 0.61.+-.0.2% is hexoseamine, 0.11.+-.0.05% is fucose and 0.23.+-.0.20 is neuraminic acid;
• (c) a sedimentation coefficient S.sub.20 W of 3.7.+-.0.3 S;
• (d) a molecular weight of 45 000.+-.5 000, determined in the ultracentrifuge;
• (e) a molecular weight of 55 000.+-.5 000 determined in sodium dodecylsulfate (SDS)-containing polyacrylamide gel;
• (f) an extinction coefficient E.sub.1 cm 1% (280 mm) of 8.1.+-.1.0;
• (g) an electrophoretic mobility in the range of the alpha.sub.1 -globulins;
• and
• (h) an isoelectric point of 4.7.+-.0.3.