Mixed phase chromatographic compositions
AssigneeVarian Associates, Inc.
InventorSeth R. Abbott
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Abstract
The mixed phase packing material of this invention, enables rapid separation of multi-sited biopolymers. This separation is achieved by "tailoring" the distance between organo-silyl groups covalently bonded to the rigid support at the Angstrom distance needed for reversible separation of the biopolymers of interest and "capping" other sterically available active sites on the support with a relatively inert compound.
Claims38
A mixed phase composition comprising a porous particle suitable for use in chromatography having a first residue covalently bonded, to less than the maximum number of sterically available support matrix active sites, said first residue being capable of separating multiple-sorbable-sited compounds, said particle also having a second residue covalently bonded to other of the sterically available support matrix active sites, said second residue being inert or substantially inert to said compounds;
- said first and second residues being directly attached to said active sites.
A process for the manufacture of a mixed phase composition comprising a porous particle suitable for use in chromatography having a first residue covalently bonded, to less than the maximum number of sterically available support matrix active sites, said first residue being capable of separating multiple-sorbable-sited compounds, said particle also having a second residue covalently bonded to other of the sterically available support matrix active sites, said second residue being inert or substantially inert to said compounds;
- said first and second residues being directly attached to said active sites;
- said process comprising the steps of:
- (a) degassing said particle,
- (b) reacting the reagent from which said first residue is formed with an aqueous slurry of said degassed particle at a time and temperature sufficient to covalently bond said first residue to said active sites;
- said particle being present in a stoichiometric excess, said excess being that of said active sites of said particle to said reagent, whereby less than the maximum number of sterically available porous particle active sites are bonded by said first residue;
- (c) reacting the product of the previous step with the reagent from which said second residue is formed, in a degassed aqueous slurry at a time and temperature sufficient to covalently bond said second residue directly to other of the sterically available porous particle active sites;
- and
- (d) recovering said mixed phase composition.
Dependencies are inferred from the claim text.