Blood coagulation factors and process for their manufacture

A method for making a hepatitis-safe preparation of at least one member selected from the group consisting of coagulation factors II, VIII, XIII, antithrombin III, and plasminogen, which method comprises first stabilizing against heat an aqueous solution containing at least one of said members by adding to said solution from 1.0 to 3.0 mols per liter of at least one amino acid selected from the group consisting of glycine, .alpha.- and .beta.-alanine, hydroxyproline, glutamine, and .alpha.-, .beta.-, and .gamma.-aminobutyric acid and from 20 to 60 w/w percent of a carbohydrate selected from the group consisting of monosaccharides, oligosaccharides, and sugar alcohols, then heating the resulting heat-stabilized aqueous solution at a temperature from 30.degree. C. to 100.degree. C. for a period of time from one minute to 48 hours at a pH from 6.5 to 8.0, whereby hepatitis virus present in said solution is inactivated, and then recovering at least one member from said solution.