Method for measuring peroxidic substances
AssigneeWako Pure Chemical Industries, Ltd.
InventorsShizuka Kasahara, Kazuhiko Yamanishi, Toshiro Hanada, Kuniaki Tokuda, Shinzo Kobatake
Life of this patent
Estimating the term…
Abstract
Peroxidic substances in a sample, e.g. one or more peroxidic substances in fats and oils or in an organic material, lipoperoxides in a living sample, etc. can be determined, directly in a sample or after extracting the lipoperoxides from a living sample with a solvent, by contacting the peroxidic substances with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color, and measuring degree of coloring colorimetrically.
Claims8
A method for determining lipoperoxides in a fluid sample obtained from a human being or other animal comprising:
- (a) extracting a lipoperoxide from a sample of serum, blood plasma or blood with a solvent selected from the group consisting of a hydrocarbon, an alcohol, a halogenated hydrocarbo dimethylformamide, dimethylsulfoxide and N-methyl-pyrrolidone;
- (b) contacting the extracted lipoperoxides with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color;
- and
- (c) measuring the degree of coloring.
Dependencies are inferred from the claim text.