Bone morphogenetic protein process
AssigneeThe Regents of the University of California
InventorMarshall R. Urist
Life of this patent
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Abstract
The process of separating bone morphogenetic protein (BMP) from bone tissue comprising the steps of demineralizing bone tissue; treating the demineralized bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for the BMP, the agent being selected from the group consisting of urea and guanidine, and thereby transforming the bone collagen to gelatin and extracting BMP into the solution of solubilizing agent; and separating the solubilizing agent and neutral salt from the solution, thereby precipitating BMP in the aqueous medium.
Claims17
The process of separating bone morphogenetic protein (BMP) from bone tissue comprising the steps of:
- (a) demineralizing bone tissue;
- (b) treating said demineralized bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for said BMP, said agent being selected from the group consisting of urea and guanidine, and thereby transforming the bone collagen to gelatin and extracting BMP into said solution of solubilizing agent;
- and
- (c) separating said solubilizing agent and neutral salt from said solution, thereby precipitating BMP in the aqueous medium.
Dependencies are inferred from the claim text.