Method of preparing polypeptides
AssigneeMax-Plank-Gesellschaft
InventorChristian Birr
Life of this patent
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Abstract
The present invention relates to a method for the preparation of polypeptides by the stepwise condensation of amino acids on a solid support material, which material comprises condensing, as a starting material, amino acids having an N-terminal of .alpha.,.alpha.-dimethyl-3,5-dimethoxybenzyloxycarbonyl, or peptide fragments which are N-terminally protected, utilizing as the support material a slightly cross-linked carrier material activated with bromoacetyl bromide with the formation of bromoacetyl phenyl groups, splitting off, in the course of the synthesis, the peptide fragments formed, separating same from contaminating by-products, and binding them again to the support material.
Claims4
Method for the preparation of a polypeptide comprising the steps of
- (a) condensing a carrier material consisting of less than 0.5% cross-linked polystyrene gel and including bromacetyl phenyl groups whereby the gel is activated with a member selected from the group consisting of peptide fragments composed of at least 3 amino-acids, isoleucine, leucine, valine, protective group bearing cysteine and protective group bearing lysine, each member having an N-terminal of alpha-alpha-dimethyl-3,5-dimethoxy benzyloxycarbonyl to protect the N-terminal and provide a means for photometric following of the reaction;
- (b) condensing the resulting carrier bound member with one or more amino-acids or peptide fragments to form a peptide moiety;
- (c) splitting off the peptide moiety;
- (d) separating the peptide from contaminating byproducts;
- (e) introducing an N-terminal of alpha-alpha-dimethyl-3,5-dimethoxybenzyloxycarbonyl into the peptide moiety;
- and
- (f) condensing the product of step (e) again with the carrier material of step (a) and repeating steps (b) to (f) until the peptide moiety, formed on the carrier material, is equivalent to the desired end product polypeptide, and thereafter splitting off the peptide moiety and isolating the desired end product polypeptide thus freed.
Dependencies are inferred from the claim text.