Separation of amino acids by liquid chromatography using chiral eluants

A process for separating enantiomers which comprises contacting the same on a solid non-chiral chromatographic support, with an aqueous solution containing a naturally occurring chiral ligand and a suitable metal ion capable of forming with the enantiomers inner sphere complexes of different stability and/or different partition coefficient.

A process for separating underivatized enantiomers which comprises contacting the same on a solid non-chiral chromatographic support, with an aqueous solution containing less than about 10 volume % organic solvent, and containing a chiral ligand and a suitable metal ion capable of forming with the enantiomers inner sphere complexes of different stability and/or different partition coefficient.

A process for separating enantiomers which comprises contacting the same, on a solid non-chiral chromatographic ion exchange column, with an aqueous solution containing a chiral ligand and a suitable metal ion capable of forming with the enantiomers inner sphere complexes of differential stability and/or different partition coefficient.

A process for separating enantiomers which comprises contacting the same on a solid non-chiral reversed phase chromatographic support, with a aqueous solution containing a chiral ligand and a suitable metal ion capable of forming with the enantiomers inner sphere complexes of different stability and/or different partition coefficient.

In a process for preparing enantiomers the improvement which comprises the separation of the optical isomers by contacting them, on a solid non-chiral chromatographic support, with an aqueous solution containing less than about 10 to 20 volume % of organic solvent, and containing a chiral ligand and a suitable metal ion capable of forming with the enantiomers inner sphere complexes of different stability and/or different partition coefficient.