Reducing light scatter in photometric measurements involving suspended particles
AssigneeContraves AG
InventorRaymond Frey
Life of this patent
Estimating the term…
Abstract
A method of reducing scattered light during photometric measurements, especially during the photometric measurement of haemoglobin and a reagent for the performance of the method. To a suspension which is ready for a measurement operation there is admixed an additive which reduces the difference between the refractive index of the suspension and the mean refractive index of the suspended phase. The reagent for the photometric determination of the haemoglobin comprises a lysing agent, means for modifying the refractive index, means for stabilizing the leukocytes and a solvent. The reagent is admixed as an additive to a suspension of blood particles in an isotonic solvent.
Claims5
A method of reducing disturbances of photometric measurement due to scattered light in a suspension consisting of a suspension agent and a suspended phase comprising suspended particles, which suspension is to be photometrically measured, said suspension agent and said suspended phase each having a respective refractive index, comprising:
- adding a chemical component to the suspension, said chemical component having a refractive index outside of the range encompassed by the refractive index of the suspension agent and the mean refractive index of the suspended phase and in a quantity effective to reduce such range.
A reagent for reducing the disturbance due to scattered light of a photometric measurement of a suspension of blood particles in an isotonic suspension agent, said reagent containing:
- a lysing agent;
- a first chemical component for modifying the refractive index of the suspension;
- a second chemical component for stabilizing the leukocytes;
- and
- a solvent capable of dissolving said lysing agent and said first and second chemical components.
Dependencies are inferred from the claim text.