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US 4,289,854 AGranted
Cell culture method and apparatus
AssigneeMonsanto Company
InventorsWilliam R. Tolbert, Mary M. Hitt, Joseph Feder
Life of this patent
Estimating the term…
Abstract
A cell culture system and apparatus is provided for the agitated suspension culturing of mammalian cells in a flask assembly in which the culture medium and cells are gently agitated by the slow rotation of agitator means suspended downwardly in the culture medium and having at least one flexible sheet with a relatively large surface area which is positioned on the agitator in a manner to allow it to billow like a sail as liquid spills from its trailing edge during said rotation of the agitator means.
Claims8
Apparatus for the suspension culturing of cells comprising
- (A) a hollow flask having an opening at the top,
- (B) shaft means within said flask and supported downwardly from said top opening, and
- (C) a rotatable agitator means supported downwardly from said shaft means, said agitator means having supported thereon at least one flexible sheet, said flexible sheet being thus supported at upper and lower positions of the sheet to permit billowing of said sheet during rotation of the agitator means.
Dependencies are inferred from the claim text.