Interferon production
AssigneeBeecham Group Limited
InventorJeffery H. Robinson
Life of this patent
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Abstract
A method for preparing interferon in which MRC-5 cells are induced for 2 to 3 hours with an interferon-inducing medium containing from 0.1 to 50 mg 1.sup.-1 of a ds-RNA, 100 mg 1.sup.-1 of DEAE-Dextran and 250 m.M of sucrose. Other sugars which can be used include glucose, galactose, fructose, mannose and maltose, and a preferred ds-RNA is naturally occurring ds-RNA isolated from P. chrysogenum.
Claims9
A method for preparing interferon, in which interferon-producing cells are contacted in vitro with an interferon-inducing medium comprising a non-toxic quantity of an interferon-inducing double-stranded polynucleotide, diethylamine ethyl dextran (DEAE-Dextran), and at least 100 m.mol 1.sup.-1 of a water soluble sugar in a physiologically acceptable aqueous vehicle at physiological conditions of temperature and pH, and the interferon so formed is thereafter separated.
Dependencies are inferred from the claim text.