Physiological preparation containing loaded cells of expanded volume in suspension, for preferential accumulation in spleen and liver

A process for preparing a mass of loaded cells suspended in a solution which cells by their loading are provided with material intended for chemical or physical interaction with substances present outside the cells, comprising the steps of suspending living animal cells, selected from the group consisting of erythrocytes, lymphocytes, thrombocytes and leucocytes having cell membranes, in a cell-compatible solution, increasing the permeability of the cell membranes by the effect of osmotic pressure, or by the effect of an electric field, or both, incorporating loading material selected from the group consisting of medicaments and radionuclides into the cells by passage of said material from a cell-compatible solution through the membranes of increased permeability, restoring the original permeability of the membranes by healing up the membranes by regeneration effect, then separating the cells from the solution in which they were suspended and putting them for preservation in suspension in a physiological solution of the same osmolarity as the loaded cell content, said process incorporating the improvement consisting in that:

• in the step of incorporating material into the cells, a first material is provided in the cell-compatible solution and is therefrom incorporated into the cells, which material is capable of interaction with substances present in the spleen or liver of an animal body and has a destructive effect on the cell membranes by interaction therewith that would lead to premature destruction of the cell membranes in the absence of agents inhibiting such effect, and there is incorporated into said cells from said solution an additional material selected from the group consisting of proteins and sugars which is capable of interacting with the material having a destructive effect on the cell membranes, by the formation of hydrogen-bridge-bonds or covalent bonds therewith, so as to prevent reaction with the membranes without impairment of the intended effect on substances present in the liver or spleen, and, further, in that
• the loaded cells formed are subjected to the step of being placed into an isotonic solution in which at least one substance of an organic nature is contained which is capable of producing a change in the cell membranes by a reaction with a lipid or protein layer of the membrane without destruction of the loaded cells, said additional material being provided in said cell-compatible solution used in said material incorporating step in such dosing, that after the performance of said incorporation step and performance of the step of restoring the original semipermeability of the loaded cells, the interaction of materials in the loaded cells with the cell membranes will be prevented for a predetermined period of time and, further, in that
• the loaded cells are left standing in said isotonic solution for such a time, and the concentration of the substance or substances capable of producing a change in the cell membranes is so selected, that when, after ther preparation of a loaded cell suspension in a physiological solution in accordance with the process said suspension is injected into the bloodstream of an animal body, the loaded cells thereof will be separated from the blood and broken down by the system of body organs composed of the spleen and liver.