Ultrasensitive enzymatic radioimmunoassay method
AssigneeUnited States of America
InventorsCurtis C. Harris, Robert Yolken, Ih-Chang Hsu
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Abstract
A method of quantitatively measuring antigen or antibody concentration by selectively coupling an antigen to a solid substrate, coupling an enzyme-conjugated antibody to the antigen and reacting a radioindicator with the enzyme-conjugate producing a radiolabelled product. The product is scintillation counted to yield the measurement. Direct or indirect coupling of the antigen to the substrate can be used. Direct or indirect coupling of the enzyme-conjugate can be used. The preferred radioindicator is adenosine-5'-monophosphate used with alkaline phosphatase. Others are presented.
Claims20
A method of quantitatively measuring the concentration of an immunosystem reactant comprising:
- (a) preparing a solid substrate;
- (b) selectively adhering the immunosystem reactant to said substrate;
- (c) binding an enzyme-conjugated antibody to said reactant;
- (d) reacting a radiolabelled indicator with said enzyme-conjugated antibody thereby producing a radiolabelled product and an unlabelled product;
- (e) separating said radiolabelled product(s) from unreacted indicator;
- and
- (f) measuring the extent of reaction by radioactive counting said radiolabelled product, said counting yielding a quantitative measurement of the concentration.
Dependencies are inferred from the claim text.