Method for the cultivation of Basidiomycetes
AssigneeKureha Kagaku Kogyo Kabushiki Kaisha
InventorsChikao Yoshikumi, Toshihiko Wada, Himromitsu Makita, Kinzaburo Suzuki
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Abstract
In submerged culture of a Basidiomycete belonging to the genus Coriolus, the seed culture to be inoculated into the medium is homogenized to form a homogenous slurry and the slurry-formed seed culture is inoculated into the medium. This improves the cultivation rate of the Basidiomycetes.
Claims1
In a method for growing a fungus Coriolus versicolor (Fr.) Quel. by inoculating a culture medium with a homogenized slurry of seed mycelia of said fungus and cultivating said mycelia under submerged conditions to produce growth of said fungus, the improvement comprising the steps of:
- treating seed mycelial lumps of said fungus with rotating propellers in a homogenizing apparatus at a speed of 3,000 to 6,000 r.p.m. for 1.5 to 2.5 minutes, thereby reducing the size of said seed mycelial lumps to 50 to 1,000 microns and at the same time obtaining homogeneously dispersed seed mycelia in the form of threads while substantially preventing cuts into said seed mycelia
- inoculating said culture medium with the thus obtained seed mycelia at an inoculating rate of 0.01 to 0.2 g of said seed mycelia per liter of said culture medium, and
- carrying out said cultivation under submerged conditions.
Dependencies are inferred from the claim text.