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US 4,288,553 AGranted
Disopyramide compounds for conjugating proteins used in immunoassays for disopyramide
AssigneeSyva Company
InventorsPrithipal Singh, Pyare L. Khanna, Floyd W. Colvin
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Abstract
Novel carboxyalkylated disopyramides (NORPACE.RTM.) are provided as precursors for conjugating proteins, either antigenic for the preparation of antibodies or enzymatic for the preparation of enzyme conjugates, which antibodies and enzyme conjugates find use as reagents in immunoassays. The combination of antibodies and enzyme conjugates provide for sensitive, accurate, rapid assays for disopyramide without interference from closely analogous compounds, such as methadone or disopyramides metabolites.
Claims12
A compound of the formula:
- (A--CONHR(CO).sub.m).sub.n Z
- wherein:
- A is of the formula:
- ##STR2## R is a saturated aliphatic linking group of from one to six carbon atoms;
- Z is hydrogen, hydroxyl, alkoxyl, a group forming an activated ester capable of amide formation in an aqueous medium, or a polypeptide which is antigenic or an enzyme;
- m is zero or one when Z is a poly(amino acid) and is otherwise one;
- and
- n is one when Z is other than a poly(amino acid) and is otherwise one to the molecular weight of Z divided by 500.
5 more elements
A compound of the formula:
- ACONHR.sup.1 COZ.sup.1
- wherein:
- A is of the formula:
- ##STR3## R.sup.1 is a saturated aliphatic linking group of from one to three carbon atoms;
- Z.sup.1 is hydrogen, hydroxyl, alkoxy of from one to six carbon atoms, or a group forming an activated ester capable of amide formation in an aqueous medium.
2 more elements
A compound of the formula:
- (ACONHR.sup.2 (CO).sub.m.spsb.2).sub.n.spsb.2 Z.sup.2
- wherein:
- A is of the formula:
- ##STR4## R.sup.2 is a saturated aliphatic linking group of from one to three carbon atoms;
- Z.sup.2 is a poly(amino acid), which is antigenic or an enzyme;
- m.sup.2 is zero or one;
- n.sup.2 is at least one and not greater than the molecular weight of Z.sup.2 divided by 1,000.
4 more elements
Dependencies are inferred from the claim text.