Ascorbate resistant composition, test device and method for detecting a component in a liquid test sample
AssigneeMiles Laboratories, Inc.
InventorsThomas A. Magers, John E. Sheats, David L. Tabb
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AssigneeMiles Laboratories, Inc.
InventorsThomas A. Magers, John E. Sheats, David L. Tabb
Estimating the term…
A composition, device and method for determining the presence of a component in a test sample which contains an interfering substance, such as ascorbic acid, are disclosed. The composition comprises an oxidase or other substance capable of producing peroxide in the presence of the component, a peroxidatively active substance, a chromogen capable of providing a detectable response (such as a color change) in the presence of peroxide and a peroxidatively active substance, and a complex of mercuric ion and one or more ligands wherein (a) the ligand binds covalently to the ion to form a water-soluble complex, and the ligand is not oxidizable by the complexed ion, and (b) the complex has a stability constant, K.sub.s, of at least 10.sup.7 and is substantially noninterfering with respect to the detectable response.
In a composition for determining the presence of a component in a test sample, wherein said composition comprises a reagent system containing a peroxidatively active substance, and an oxidation-reduction indicator capable of providing a detectable response in the presence of H.sub.2 O.sub.2 and said peroxidatively active substance, and wherein said response is suceptible to being adversely influenced by the presence in said test sample of ascorbic acid, said composition further comprising a heavy metal compound which, in its ionized state, has an oxidation potential below that of said indicator, but above that of ascorbic acid,
Dependencies are inferred from the claim text.