Test method and composition therefor
AssigneeBaxter Travenol Laboratories, Inc.
InventorsErnest V. Groman, Marian E. Sacco
Life of this patent
Estimating the term…
Abstract
Test methods in which an enzyme conversion of at least one member of a family of compounds to an analyte precedes an immunoassay of the analyte are improved by conducting the enzyme conversion step substantially simultaneous with the immunoassay. A novel composition is provided for conducting the improved test method.
Claims40
In a specific binding assay for the determination of the total amount of steriod present in a test sample containing steriod and steriod conjugates, wherein the improvement comprises, contacting the test sample substantially simultaneously with receptor for the steriod, and with an enzyme composition suitable for converting the steriod conjugates to the steriod under conditions suitable for the conversion of the steriod and the binding of steriod by the receptor.
In a radioimmunoassay for the determination of total estriol in a test sample containing free estriol and estriol conjugates, wherein the improvement comprises, contacting the test sample substantially simultaneously with a receptor for estriol and with an enzyme composition suitable for converting the estriol conjugates at a pH from about 4 to about 6 and under conditions suitable for the conversion of the estriol conjugates and the binding of estriol by the receptor.
In a specific binding assay for the determination of total estriol and estriol conjugates, wherein the improvement comprises, contacting the test sample substantially simultaneously with receptor for estriol and with an enzyme composition suitable for conversion of estriol conjugates to free estriol under conditions suitable for the conversion of the estriol conjugates and for the binding of the estriol by the receptor.
A composition for the determination of the total amount of steriod and its conjugates in a test sample consisting essentially of a receptor for the steriod and an amount of enzyme composition suitable for converting the steriod conjugates to the steriod.
Dependencies are inferred from the claim text.