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US 4,288,425 AGranted
Method and apparatuses for electrophoretic immunoassay
AssigneeTechnicon Instruments Corporation
InventorsMartin J. Lee, Leonard Ornstein
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Abstract
Methods and apparatuses are featured for preconcentrating immunological reactants prior to their contact and reaction, to greatly enhance the rate of reaction and the sensitivity. The preconcentration is accomplished within the reaction medium and is followed automatically by the separation of unreacted and reacted reactants resulting in a simplified and compact apparatus.
Claims10
A disc-electrophoretic method for analyzing a sample constituent, said constituent having an ionic charge, said method comprising the steps of:
- (a) introducing said sample constituent into a porous medium;
- (b) applying an electric field across said medium for causing said constituent to migrate within said medium and concentrate by steady-state stacking in a first position thereof;
- (c) further migrating said stacked concentrated constituent through a second portion of said medium containing a reactant, so as to cause a reaction therewith;
- and
- (d) measuring said reaction.
2 more elements
Dependencies are inferred from the claim text.