Polyfunctional epoxides and halohydrins used as bridging groups to bind aromatic amine group-containing alcohols and thiols to hydroxyl bearing substrates
OwnerBrian S. Seedinventor
Life of this patent
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Abstract
A process for preparing a derivative of an hydroxyl bearing substrate by reacting an hydroxyl bearing substrate with oxirane bearing molecules to produce an oxirane ring bearing substrate; and reacting the oxirane ring with a substituted primary aromatic amine selected from primary aromatic amine alcohols and primary aromatic amine thiols. Among the compositions so produced is a stable modified hydroxyl bearing substrate or hydroxyl bearing sheet having covalently bonded thereto primary aromatic amines which can be diazotized. The diazotized substrate selectively, covalently binds proteins, polypeptides, peptides, nucleic acids, RNA, single stranded DNA, and nucleic acid hybrids.
Claims33
A stable modified hydroxyl bearing substrate comprising substituted phenyl thioether groups having structural units having the general formula:
- ##STR5## in which HBS comprises a hydroxyl bearing substrate, R is selected from the group consisting of alkyl groups, hydroxyl bearing alkyl groups, aryl groups, alkylaryl groups, alkyl ether groups, hydroxyl bearing alkyl ether groups, aryl ether groups, alkylaryl ether groups, and hydroxyl bearing alkylaryl ether groups;
- and in which Z is selected from the group consisting of primary amines, diazo chlorides, diazo fluoborates, and diazo fluorophosphates.
A stable modified hydroxyl bearing sheet comprising substituted phenyl groups having structural units having the general formula:
- ##STR6## in which HBS comprises a hydroxyl bearing substrate, R is selected from the group consisting of alkyl groups, hydroxyl bearing groups, aryl groups, alkylaryl groups, alkyl ether groups, hydroxyl bearing alkyl ether groups, aryl ether groups, alkylaryl ether groups, and hydroxyl bearing alkylaryl ether groups;
- Y is selected from the group consisting of oxygen and sulfur;
- and in which Z is selected from the group consisting of primary amines, diazo chlorides, diazo fluoborates, and diazo fluorophosphates.
A process for preparing a derivative of a hydroxyl bearing substrate comprising the steps of:
- reacting a hydroxyl bearing substrate with oxirane bearing molecules to produce an oxirane ring bearing substrate;
- and
- reacting the oxirane ring bearing substrate with a substituted primary aromatic amine selected from the group consisting of primary aromatic amine alcohols and primary aromatic amine thiols.
Dependencies are inferred from the claim text.