Process for isolating active debriding agent from bromelain
AssigneeRiker Laboratories, Inc.
InventorWilliam Galbraith
Life of this patent
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Abstract
A process is disclosed for isolating an enzyme mixture useful in the treatment of devitalized tissue from crude bromelain. The process comprises the steps of: (1) suspending the crude bromelain in a weakly basic buffer, preferably sodium borate buffer, to selectively dissolve the active enzyme mixture; (2) separating the undissolved solids from the solution; and (3) removing small molecules having a molecular weight of 10,000 or less from the solution.
Claims15
A process for isolating a product from crude bromelain useful in debriding divitalized tissue in mammals comprising the steps of:
- A. suspending the crude bromelain in a solution of a weakly basic buffer having a pH of about 8 to 10.5 to selectively solubilize the active components;
- B. separating the undissolved solids from the solution;
- and
- C. removing a substantial portion of the small molecules having a molecular weight of about 10,000 or less from said solution.
A process for isolating a product from crude bromelain useful in debriding devitalized tissue in mammals comprising the steps of:
- A. suspending the crude bromelain in a solution of a weakly basic buffer having a pHa of about 8 to 10.5 to selectively solubilize the active components;
- B. separating the undissolved solids from the solution;
- C. removing a substantial portion of the small molecules having a molecular weight of about 10,000 or less from said solution;
- D. removing the precipitate formed in step C from the resulting solution;
- E. concentrating said resulting solution;
- and
- F. lyophilizing said resulting solution.
Dependencies are inferred from the claim text.