Method for producing thrombolytic preparation
AssigneeThe Green Cross Corporation
InventorTadakazu Suyama
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Abstract
A method for producing a thrombolytic preparation characterized in that among treatment conditions in the urokinase production pH is maintained within the neutral or weakly alkaline range throughout each treatment step to produce a thrombolytic preparation containing urokinase having a molecular weight of 54,000.+-.10,000 as major ingredient.
Claims3
In a method for producing a thrombolytic preparation from a fresh urine or a crude urokinase solution which consists essentially of (1) adsorbing urokinase on a selected adsorbent, (2) eluting and recovering the absorbed urokinase, (3) heat-treating the recovered urokinase solution at a temperature of 50.degree. to 70.degree. C. for 8 to 12 hours, and (4) subjecting the heat-treated solution to dialysis, the improvement comprising carrying out the heat-treatment step at pH 6 to 8 and carrying out the dialysis step at pH 5.5 to 12 to recover urokinase having a molecular weight of 54,000.+-.10,000.
Dependencies are inferred from the claim text.