Polysaccharide and bacterial fermentation process for its preparation
AssigneeMerck & Co., Inc.
InventorsKenneth Suk Kang, George T. Veeder, III, Danny D. Richey
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Abstract
A process for producing a heteropolysaccharide by a bacterial fermentation procedure in which a species of bacteria or a mutant thereof is incubated in a fermentation medium which contains a carbon source, preferably a hydrolyxed starch, a source of magnesium ions, a source of phosphorous, a source of nitrogen and water with the incubation taking place at a temperature of about 28.degree. to about 35.degree. C.
Claims8
The process for preparing Heteropolysaccharide 10, the heteropolysaccharide containing about 3% protein and about 97% carbohydrate, the carbohydrate portion of which contains about 19% of a uronic acid, about 39% glucose, about 29% galactose and about 13% fucose, said heteropolysaccharide being compatible with Methylene Blue chloride dye, that comprises cultivating under submerged aerobic conditions at a temperature of about 28.degree. to about 35.degree. C. a heteropolysaccharide producing strain of Klebsiella pneumoniae or a heteropolysaccharide producing mutant thereof in an aqueous nutrient medium containing as a source of carbon hydrolyzed starch having a dextrose equivalent of about 20-35, a source of nitrogen, a source of magnesium and a source of phosphorus, until substantial viscosity has been imparted to said medium, and recovering the heteropolysaccharide.
An aqueous nutrient medium containing a Heteropolysaccharide 10 producing strain of Klebsiella pneumoniae or a heteropolysaccharide 10 producing mutant thereof and as a source of carbon hydrolyzed starch having a dextrose equivalent of about 20-35, a source of nitrogen, a source of magnesium and a source of phosphorus.
An aqueous fermentation medium containing a strain of Heteropolysaccharide 10-producing Klebsiella pneumoniae or a heteropolysaccharide 10-producing mutant thereof, as a source of carbon hydrolyzed starch having a dextrose equivalent of about 20-35, a source of nitrogen, a source of magnesium and a source of phosphorus, and heteropolysaccharide 10 produced by the strain or the mutant.
Dependencies are inferred from the claim text.