E. coli enterotoxin vaccine for veterinary and human use
AssigneeMerck & Co., Inc.
InventorsGuadalupe A. Limjuco, Yashwant D. Karkhanis, Dennis J. Carlo
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Abstract
A vaccine against E. coli caused diarrhea is prepared from an isolate of the homogeneous heat-labile enterotoxin of human strain MB3353 of Escherichia coli, ATCC 31361, or of the porcine strain MB3708, ATCC 31362. The toxin, which is a protein, is a low molecular weight species of 10,000-13,000.
Claims2
A method of immunizing against E. coli induced diarrhea comprising administering to humans or animals from 25 .mu.g to 1,000 .mu.g of the enterotoxin isolated from E. coli culture filtrate having a molecular weight of 10,000-13,000 when determined by gel filtration or by sucrose density gradient;
- containing 90% protein, 2% hexose, no 2-keto deoxy octonic acid;
- being a homogeneous single chain protein with N-terminal alanine when determined by the dansylation method and SDS-acrylamide electrophoresis;
- and showing no activity in the limulus lysate assay.
Dependencies are inferred from the claim text.