Process for the preparation of CoA-SPC from Bakers' yeast
AssigneeResearch Corporation
InventorsEdsel T. Bucovaz, John C. Morrison, Walter D. Whybrew, Stanley J. Tarnowski, Jr.
Life of this patent
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Abstract
A process for preparing CoA-SPC Bakers' yeast extract which comprises: lysing Bakers' yeast cells; separating the Bakers' yeast cell lysate into solid and supernatant fractions wherein said solid fraction is substantially free of t-factor; treating said solid fraction to solubilize insoluble proteinaceous material other than the insoluble CoA-SPC; separating said solubilized proteinaceous materials from the fraction containing said insoluble CoA-SPC; and contacting said fraction containing said insoluble CoA-SPC with said supernatant fraction containing t-factor to produce soluble CoA-SPC.
Claims18
A process for preparing CoA-SPC Bakers' yeast extract which comprises:
- lysing Bakers' yeast cells;
- separating the Bakers' yeast cell lysate into solid and supernatant fractions wherein said solid fraction is substantially free of t-factor;
- treating said solid fraction to solubilize insoluble proteinaceous material other than the insoluble CoA-SPC;
- separating said solubilized proteinaceous materials from the fraction containing said insoluble CoA-SPC;
- contacting said fraction containing said insoluble CoA-SPC with said supernatant fraction containing t-factor to produce soluble CoA-SPC;
- and
- wherein said t-factor is characterized by that fraction of Bakers' yeast having a molecular weight of 400 to 1000 and which solubilizes insoluble CoA-SPC in the presence of chloride ions.
The process for the preparation of CoA-SPC Bakers' yeast extract which comprises:
- (a) drying Bakers' yeast;
- (b) grinding the dried Bakers' yeast;
- (c) hydrating said ground dried yeast in water to form a suspension;
- (d) separating said suspension into solid and supernatant fractions wherein said solid fraction is substantially free of t-factor;
- (e) treating said solid fraction to solubilize insoluble proteinaceous material other than the insoluble CoA-SPC;
- (f) separating said solubilized proteinaceous materials from the fraction containing said insoluble CoA-SPC;
- (g) contacting said fraction containing said insoluble CoA-SPC with a source of t-factor to produce insoluble CoA-SPC;
- (h) solubilizing and recovering the CoA-SPC;
- and
- wherein said t-factor is characterized by that fraction of Bakers' yeast having a molecular weight of 400 to 1000 and which solubilizes insoluble CoA-SPC in the presence of chloride ions.
Dependencies are inferred from the claim text.