Microbial lipase, process for its preparation and microbiologically pure culture therefor
AssigneeMeito Sangyo Kabushiki Kaisha
InventorsYoshitaka Kokusho, Haruo Machida, Shinjiro Iwasaki
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Abstract
A microbial lipase which has (i) an optimal pH for activity of about 9.+-.0.5, (ii) an optimal temperature for activity of about 40.degree. C. to about 48.degree. C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30.times.10.sup.4 to about 40.times.10.sup.4 ; a process for its preparation; and microbiologically pure culture therefor.
Claims11
A microbial lipase which has
- (i) an optimal pH for activity of about 9.+-.0.5,
- (ii) an optimal temperature for activity of about 40.degree. C. to about 48.degree. C.,
- (iii) a lipase activity to be activated by bile salts,
- (iv) a cholesterol esterase activity, and
- (v) a molecular weight of about 30.times.10.sup.4 to about 40.times.10.sup.4.
A biologically pure culture of Alcaligenes sp. PL-679 Meito, ATCC 31371 strain which has the ability to produce a microbial lipase capable of being activated by bile salts and characterized by having
- (i) an optimal pH for activity of about 9.+-.0.5,
- (ii) an optimal temperature for activity of about 40.degree. C. to about 48.degree. C.,
- (iii) a lipase activity to be activated by bile salts,
- (iv) a cholesterol esterase activity, and
- (v) a molecular weight of about 30.times.10.sup.4 to about 40.times.10.sup.4,
- by aerobic fermentation in a nutrient culture medium containing a carbon source, a nitrogen course and minerals at a pH of about 5.5 to about 11 and a temperature of about 5.degree. to about 40.degree. C.
Dependencies are inferred from the claim text.