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US 4,283,389 AGranted
Novel antibiotic, BN-183B substance
AssigneeMeiji Seika Kaisha, Ltd.
InventorsNorio Ezaki, Shinji Miyadoh, Yasuaki Ogawa, Takashi Hisamatsu, Harumi Fukuyashu, Yujiro Yamada
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Abstract
A novel antibiotic designated BN-183B substance is disclosed which is produced by cultivating a strain of the genus Pseudomonas. The antibiotic has a strong action against Gram-positive and Gram-negative bacteria.
Claims2
An antibiotic designated BN-183B substance whose hydrochloride has the following characteristics or a pharmaceutically acceptable salt thereof
- (1) Elemental analysis values:
- C 39.84%, H 5.25%, N 6.43%, Cl 22.90%
- (2) Molecular weight:
- 383
- (3) Molecular formula:
- C.sub.14 H.sub.20 N.sub.2 O.sub.6 Cl.sub.2.HCl
- (4) Melting point:
- begins to turn brownish at 190.degree. C. and to foam and decompose at 214.degree. C.
- (5) Ultraviolet absorption spectrum:
- shown in FIG. 1
- (6) Infrared absorption spectrum:
- Absorption bands at 3400, 3020, 1680, 1620, 1570, 1520, 1400, 1360, 1330, 1270, 1240, 1180, 1160, 1130, 1090, 1080, 1020, 930, 880, 850, 830, 800, 740, 700 cm.sup.-1 and absorption spectrum as shown in FIG. 2.
- (7) Specific rotation:
- [.alpha]sub.D.sup.23 -9.degree. (c=1, water)
- (8) Solubility:
- very soluble in water, slightly soluble in methanol, and scarcely soluble in acetone, chloroform and ethyl acetate
- (9) Color reactions:
- Positive:
- ferric chloride, ninhydrin and Fehling
- Negative:
- Molish and biuret
- (10) Distinction by neutrality, acidity and basicity:
- behaves as a basic substance in filter paper electrophoresis
- (11) Appearance:
- white to slightly yellowish powder
- (12) Rf values in thin-layer chromatography:
- Butanol-acetic acid-water (2:2:1):
- 0.66
- Ethyl acetate-acetic acid-water (60:17:17):
- 0.34
- Butanol-pyridine-acetic acid-water (6:4:1:3):
- 0.65.
29 more elements
Dependencies are inferred from the claim text.